James, Only help I can be is to tell you that we have had a Calibur with a red diode laser for about three years (I'm not sure who the supplier is). The Calibur is used all day every day (the red laser is used lots, but not all the time). In three years, we have not had to have the red laser (or the argon laser, for that matter) replaced. It still seems to be working fine. One point is that we try to be careful to turn the red laser off manually when we are finished with a four color protocol. Otherwise it will stay on unnecessarily until someone invokes instrument settings that turn it off. I have been told that this extra use is worse for it than extra on-ing and off-ing. Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
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