Donghui, We are a very busy multiuser flow cytometry core facility. We have had our AutoMACS for about 1 year now and are absolutely delighted. We use it to sort about 2-3 times each day. To say the least it has taken a lot of pressure off of the sorting flow cytometers. Before AutoMACS we had a SuperMACS system. I have found the AutoMACS to be extremely easy to use and the results have been just as good if not better than the SuperMACS. I can teach someone how to use the device in about 10 minutes compared to more than 1 hour for the SuperMACS. As a core we supply all the needed reagents. Also, we keep the unit in a hood to keep the cells sterile. In the last year we have had one small problem with a valve leaking. Miltenyi sent us a replacement AutoMACS the next day, they repaired our unit and sent it back to us. We lost no experiments. Other then this 1 incident, there have been no other maintenance issues. I too would be interested in documenting usage in some sort of fool proof way. Right now we have to rely on the researchers to put their name in a scheduling book and I am sure we lose some people along the way. Does some one make a device that would turn the electricity on to the AutoMACS only after they enter an electronic code? Good luck. Glenn ************************************************* Glenn Paradis MIT Flow Cytometry Core Facility E18-463 Massachusetts Institute of Technology Cambridge, MA 02139 (617)253-6454 lab (617)452-4132 office gap@mit.edu http://web.mit.edu/flowcytometry/www/ ************************************************* >Dear Flowers, > >Could people with autoMACS (Miltenyi Biotec) comment on use of the >instrument, ease of operation, maintenance, etc. What is the most >important thing for a shared instrument among multiple users, any >way of keeping track of usage. > >Thanks for any input. > >Donghui Cheng >Iowa State University -- Glenn Paradis
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