RE: FACS question

From: Donnenberg, Albert (donnenbergad@MSX.UPMC.EDU)
Date: Mon Dec 17 2001 - 11:51:01 EST


Paul and colleagues-

With due respect to the flow wizards,  I think we are being WAY to nitpicky
here.  The single assumption necessary to make the original Rhodamine 123
question perfectly intelligible is that the questioner is using a commercial
4-color analytical cytometer with filters as supplied by the manufacturer.
For the purpose of publication, we have always thought it sufficient to
state that model of the analytic cytometer used to collect the data.  From
there, FL1, FL2 etc. need no further interpretation.  In the case of
sorters, where user configured optics are more common, it is worth the ink
to specify the filter configuration.

Albert Donnenberg



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