Dear All; I am a beginner regarding to the operation of MoFlo Cytometer. Could anyone tell me what I could do to solve a problem that I am facing and feeling frustrated about? The problem is that I do not have trigger signals from Flow check after I got my stream straight, ~{!.~}annular ring~{!/~} and beads signal shown through the top pinhole. If I raised the nozzle, I sometimes got a very weak signal from FL1 vs FL2 but FSC vs SSC. When I took off the tube, which connects the universal tower, I saw that laser beam is directed above the hole of the universal tower. Any suggestions? Thanks in advance. Xin Zhan
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