I don't think anyone was being harsh. I'm a flow novice and one thing I learned first about flow people is that they are the most helpful people in the business and they have a sense of humor. Anyone who's read Howard's book should know that. Remember the "no Falcon around" line? If we were thin-skinned and arrogant, we wouldn't be Flow People. We might be Study Section members though,... but I'm digressing. I say keep up the banter and don't look for excuses to be offended. Dan Rosson Ph.D. Lankenau Institute for Medical Research 100 Lancaster Ave. Wynnewood PA 19096 -----Original Message----- From: Gerstein, Rachel [mailto:Rachel.Gerstein@umassmed.edu] Sent: Thursday, December 13, 2001 9:21 AM To: cyto-inbox Subject: RE: FACS question Aren't you being a bit harsh? I want to suggest that we all try to phrase our responses in a manner that educates, but does not flog the person asking the question. Yes, its really important to ask the right question, with a request for specific information. Yes, we all grind our teeth in response to some questions. Really poorly phrased questions tend not to get answered. However, there have been some very harsh responses in the last few weeks. We all were new and/or ignorant at some point. Lets not scare away people who might really benefit from the list ! just mho... Rachel ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: J.Paul Robinson > Reply To: jpr@flowcyt.cyto.purdue.edu > Sent: Tuesday, December 11, 2001 9:20 PM > To: Cytometry Mailing List > Subject: Re: FACS question > > > Colleagues: I am going to answer this question in a manner that I hope you > all > understand (or not)......(think of the bad data issue) > > You can solve the problem by adding reagent A to reagent B, plotting FL3 > Vs FL2. > If you add reagent C, then a single histogram of FL5 should do the trick. > I think you > could also try probe A and probe B, both of which should have the right > spectra...Oh, > turn the power of laser 1 up a bit, and you should get some valuable > data..... > > sorry, I couldn't resist it... > please stop using terms which are totally undefined.... > > Paul Robinson > > > On 10 Dec 2001, at 11:58, Laura H > odges wrote: > > > I'd like to post a question on your website: > > > Can anyone suggest another P-gp accumulation probe > other than rhodamine 123 which has too broad of an > emission spectrum for my use. I am trying to find a > P-gp probe that can be used with other fluorescent > markers in channels FL2, FL3, and FL4 in a multicolor > assay. > > > __________________________________________________ > Do You Yahoo!? > Send your FREE holiday greetings online! > http://greetings.yahoo.com > J.Paul Robinson, PhD PH:(765)4940757 > Professor of Immunopharmacology > Professor of Biomedical Engineering > Purdue University FAX:(765)4940517 > EMAIL:jpr@flowcyt.cyto.purdue.edu > WEB: http://www.cyto.purdue.edu > >
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