Hello, I am just returning to my office after some travel and picked this one-week old question. During a recent meeting I heard about a new product from a joint venture between QiaGen and BD. This product is named PAXgene Blood RNA System manufactured by PreAnalytix . It is described as a special collection tube. The manufacturer claims that the blood samples can be stored for up to 5 days at room temperature. I have never used this product. Just my two cents. Michel Canton, PharmD Managing Director BioCytex France www.biocytex.fr Fang-yao Stephen HOU <shou@uci.edu> le 06/12/2001 02:56:11 Pour : Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc : (ccc : MailingList/general/Biocytex) Objet : whole blood preservative for future sorting & mRNA detection Hello, A collaborator came to me to find out information about the right preservative for whole blood. They are thinking to simply add the preservative to whole blood and ship this sample to the central processing place for lymphocyte staining, sorting and subsequent determination of cytokine mRNA levels from different subsets. The ideal preservative will stop the physiology right away and prevent the mRNA degradation. Hopefully, by using this preservative, they will be able to minimize the ex-vivo manipulation and the quantitation of mRNA will reflect the situation in vivo. I appreciate your feedback regarding this issue. Thanks. Fang-Yao Stephen Hou, Ph.D. Flow Cytometry Specialist UC-Irvine
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