I totally agree with Maryalice. We have tried for many years to do this assay both in a clinical and research setting and found similar limitations. Since we do much with CLL we also tried it in these cases as a potential control and found positive staining..the bottom line is that the antibodies available are not reliable enough for a clinical flow test at this time. Jonni >From: "Stetler-Stevenso, Maryalice (NCI)" <stetler@mail.nih.gov> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: RE: Cyclin D1 >Date: Fri, 7 Dec 2001 09:11:40 -0500 >X-PMFLAGS: 570949760 0 1 24371.cnm >Status: RO > >No matter what you read it is impossible to do Cyclin D1 by flow with >available antibodies. We tried and tried (despite having been told it >doesn't work by another very well known flow person) and found that the >antibody picks up something else that must be destroyed by fixation and >paraffin embedding. Since then I have heard from other excellent labs that >they tried and tried and couldn't do it. You can get staining in mantle cell >lymphoma but you can also get it in CLL- garden variety CLL. Now I could >rush out and write another bad flow paper about cyclin D1 in CLL that can >only be detected by flow (negative on sections) but I know that is bogus. It >is not specific by flow and a non-specific cyclin D1 is worthless. > >Maryalice Stetler-Stevenson, M.D., Ph.D. >Chief, Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH > >Sometimes you're the windshield, sometimes you're the bug. > >> ---------- >> From: Shields, Dottie >> Sent: Wednesday, December 5, 2001 1:53 PM >> To: Cytometry Mailing List >> Subject: Cyclin D1 >> >> >> Is anyone doing Cyclin D1 by Flow Cytometry? We are interested offering >> the >> test but can only find very labor intensive procedures that take at least >> two days to complete. Is anyone getting good results with Cyclin D1 by >> Flow? >> >> Thank you for your help, >> >> Dottie Shields, MT(ASCP) >> Flow Cytometry Supervisor >> Fox Chase Cancer Center >> 7701 Burholme Ave. >> Philadelphia, PA 19111 >> phone 215-728-2492 >> fax 215-728-2899 >> >> >> >> -- Jonni S. Moore, Ph.D. Associate Prof. of Path. and Lab. Med. Director of Clinical and Research Flow Cytometry University of Pennsylvania Cancer Center University of Pennsylvania School of Medicine 203 John Morgan Bldg. Philadelphia, PA 19104-6082 Phone: 215-898-6853 FAX: 215-898-4227
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:42 EST