Hi, mouse CD8 works fine, we (any many others) use the 53-6 clone, widely available. ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Richard Peppler > Sent: Wednesday, November 28, 2001 10:30 AM > To: Cytometry Mailing List > Subject: Trypin-resistant surface antigen > > > > I have a colleague who would like to be able to express a cell surface > marker protein in transfected cells that would survive enzymatic > dissociation by trypsin and collagenase and be useful for cell analysis > and > cell sorting by FACS. > > I wonder if anyone might have any suggestions as to a trypsin-resistant > cell surface antigen for which commercial Ab are available? > > I will certainly appreciate any ideas or pointers that you might be able > to > share with me. > > Rick Peppler > Research Specialist > Flow Cytometry Core Facility > Medical University of South Carolina > > > > > >
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