Hi Rick, e.g. EGFR is relative insensitive to trypsin treatment and thus could work for your application. Unfortunately the receptor is functionally active and will affect cell functions. Possibly one could inactivate or remove the cytoplasmatic kinase domain. Alternatively, the CD3 receptor has been used for such purposes (if I remember correctly) but I donīt know if this molecule is resistant to trypsin. Best regards Georg pepplerr@musc.edu am 28.11.2001 16:30:01 An: cytometry@flowcyt.cyto.purdue.edu Kopie: Thema: Trypin-resistant surface antigen I have a colleague who would like to be able to express a cell surface marker protein in transfected cells that would survive enzymatic dissociation by trypsin and collagenase and be useful for cell analysis and cell sorting by FACS. I wonder if anyone might have any suggestions as to a trypsin-resistant cell surface antigen for which commercial Ab are available? I will certainly appreciate any ideas or pointers that you might be able to share with me. Rick Peppler Research Specialist Flow Cytometry Core Facility Medical University of South Carolina
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