At 06:05 AM 11/20/01 -0800, you wrote:
>
>Dear group,
> Hi,
> Currently we are having a BD FACS Vantage with a
>488nm air cooled laser and four FL channels. We would
>like to purchase a UV laser (most importantly with a
>air cooled UV laser, there is an option with BD where
>they have suggested a heat exchanger so that one
>dosent require to use a continious source of water).
> What I wanted to inquire is if any one has gone for
>such an upgradation and what are their experiences and
>what kind of options are available (cant give any
>details of the experiments planned with the UV laser
>as someone else in our institute is going to use it
>and frankly no experiments have yet been decided apart
>from chromosome sorting). Is it possibel to use the
>machine as 2 lasers and 6 FL channels. In the BD tech
>manual I found some configurations but some how they
>didnt seem to be optimum utilization of the lasers.
> Thanks for any suggestions,
> Regards,
> Rana Nagarkatti
> SRF, Center for Biochemical Technology
> Mall Road Delhi,
> India.
>
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Hi, Rana,
I have no experience with air-cooled UV lasers, but if you are going
to sort chromosomes you must have a laser that can generate at least 300
mWatts of UV (as well as 300 mW of 457)and it should be stable. It is my
understanding that the air-cooled varieties cannot make enough light for
chromosomes. We are sorting chromosomes on a regular basis and I will even
use as much as 350 mWatts of UV. We have kryton lasers, water-cooled, that
can do this. Most water-cooled lasers are pricey but if you have many
applications for UV (or other wave-lengths), a water-cooled laser is the
way to go. As far as setting up your cytometer, have your engineer help
you understand how the PMTs see signals off of each laser that you use.
Kathy
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