Bunny, I have an LSR and, like you, am swamped with bench work. I DID NOT find your original e-mail to be offensive in any way, to any body or to the machine. The members of this list should not be replying to obviously honest messages for help in any negative way. As to your side scatter problem, it is a small draw back of the LSR in that the side scatter is reflected from another dichroic and, as BD stated in their training class, is not as sensitive or clean as on any of their other instruments. This said, I have not had much problem eventually gettting my SSC to look similar to the Calibur, but it does take some tweaking. Once you have it set, it stays very stable and reliable. Randy T. Fischer NIH/NIAMS Building 10, Room 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: bunny > Reply To: bunny@cotleur.com > Sent: Tuesday, November 13, 2001 10:36 AM > To: Cytometry Mailing List > Subject: BD LSR query > > > To all those who were offended by the fact that I posted a > query on the LSR: > there was never any attempt to "slam" this machine. In fact, > I like it a lot. I also did not imply that the machine was > not set up right. In fact-the tone of my entire post was: > what am I doing wrong? > I wish I had the time to sit in the flowlab next to a phone > for tech support-I'm sure many questions would have been > answered. Currently I am swamped with bench work and the > only time I have to address these issues is the dead of the > night. So I chose this list to "pick some brains" for > advice until I had more time to sort it out. I will > continue to post questions to this list-isn't that what it's > all about? > Again- there was not a HINT of a negative tone toward the > LSR or its manufacturer. > And to everyone who replied with helpful hints- thanks so > much! I have about 6 different things to check over now (in > MY SETUP). Thanks for the suppport. > > -- > Bunny Cotleur > Cleveland Clinic Foundation > Neurosciences NC30 > 9500 Euclid Avenue > > Cleveland, OH 44195 > > (216) 444-1164 > cotleua@ccf.org > >
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