Hello, Having not done flow cytometry before I am looking for general advice on using flow cytometry on isolated mitochondria (any references protocols etc welcome). More specifically, I wanted to measure membrane potential with rhodamine 123 and at the same time determine mitochondrial glutathione levels using monochlorobimane. Are these the best markers to go for? On a different topic, is it possible to use FACS to separate neurons from astrocytes (to then get mitochondria from the different cell types)? Or do neurons die rapidly too rapidly when their axons and dendrites are disrupted? Look forward to hearing from people soon! Regards, Diane Lee __________________________________________ Dr Diane Lee Department of Medical Biochemistry School of Medicine Flinders University GPO Box 2100 Adelaide S.A. 5001 email: diane.lee@flinders.edu.au Tel : (08)8204 4842/4945 (lab/office) Fax : (08)8374 0139
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