Dear Flowers, Anybody has an idea why can I measure approximately 50 % or more decrease in the CD34+ cell concentration of bone marrow mononuclear cell fraction or PBSC after thawing ? I use TruCount tubes from BD, but I have almost the same results using "lyse no wash" method and calculation the CD34+ concentration from the percentage and white cell count. Thanks, Pal Jakso University of Pecs Faculty of Medicine Dept. of Pathology 12. Szigeti str. Pecs 7643, Hungary
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