Re: apoptosis/cell cycle

From: Derek Davies (daviesd2@icrf.icnet.uk)
Date: Tue Nov 06 2001 - 02:23:09 EST


Hi Rachel,

I dont know about Rhodamine but I generally use Annexin-Cy3 (from MBL)
when I want to use FITC or GFP as well. Alternatively if you have a red
light source the Annexin-AlexaFluor647 from Molecular Probes
(www.probes.com) works well as well.

Derek

On 2 Nov 2001, Rachel O. Johnson wrote:
>  We are trying to look at Annexin staining along with anti-PCNA to
> study the cell cycle and apoptosis at the same time. But both
> reagents are FITC labeled. Does anyone make Annexin labeled with
> Rhodamine?

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Derek Davies                       Voice: (44) 020 7269 3394
FACS Laboratory,                   FAX: (44) 020 7269 3100
Imperial Cancer Research Fund,     e_mail: derek.davies@icrf.icnet.uk
London, UK			   mobile: 07790 604112

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