stach granules and PI

From: joan Kalnitsky (jkalnits@vt.edu)
Date: Fri Nov 02 2001 - 15:59:34 EST


I have a client who is looking at ploidy levels in tomato plants.  When we
run her plant tissue through we get nice results.  She has tried to stain
some of her tissue culture cells and the histograms get pretty ugly.  She
was wondering if starch granules take up PI and if this might be why the
nice clean peaks start looking like mounds.
	Any ideas on this one.	I could not help with that question, but told her
I would pass it on to the group.
	Thanks in advance for any and all input.
	Joan K


	Flow Cytometry Lab Supervisor
	VMRCVM
	(540) 231-4115
	FAX 540-231-7367
	jkalnits@vt.edu

	"It is better to serve than to receive."
	   B. Borg



This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:37 EST