Hi, Has anyone looked at filipin staining on a fluorescence microscope using standard dichroics (filipin excites around 350 and emits at wavelengths similar to FITC) - specifically, if you use the UV dichroic (for 350 excitation) will you see any fluorescence in the 515 to 545 range? I was told by an investigator that Olympus (I believe that's the manufacturer they spoke with) quoted a price of around $800 for a dichroic that would allow you to visualize filipin fluorescence, but they were hopeful that they might see at least some of that fluorescence with the UV dichroic. I haven't been able to find an emission spectrum for filipin. Also, thioflavin? I believe it excites around 430 and emits similarly to FITC. Again, I haven't seen an excitation spectrum and wonder if it would excite using the FITC dichroic. They aren't staining for filipin and thioflavin simultaneously (I hope). Any information on seeing fluorescence from these two compounds on a standard fluorescence microscope with standard dichroics will be greatly appreciated. Thanks. Ray Hester Univ. of South Alabama rhester@jaguar1.usouthal.edu
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