---------------------------------------------------------------------- hi, i have a basic question about flow cytometry which you might be able to advise me on! i look at t cell changes after injury and want to stain cells for cd4, ki67 and tcr-vbeta simultaneously with three different colours. should i collect a. 50,000 total events b. 20,000 cd4 events? c. 10,000 cd4+, ki67+ events? in other words, at what point in analysis is it useful to have a fixed number of cells between comparison groups? will greatly appreciate any help you can offer! thank you, liz purcell epurcell@rics.bwh.harvard.edu (LIZ PURCELL)
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