Hey ya'll, We have mouse peritoneal exudate cells that we are trying to stain with Mac3 PE, MHCII PE, CD45 APC and CD3 FITC (not all at the same time). We get a huge autofluorescent population (unstained cells) straight out of the mouse that shows up on FL1 vs. FL2 histogram. We cannot compensate this out. Our voltages are reasonable (between 450 and 550). I will take any advice. Thanks, Julie Julie G. Nelson Research Coordinator Flow Cytometry Facility Center for Tropical and Emerging Global Diseases University of Georgia
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