RE: intracellular IL-5 vs IL-4

From: Swartzendruber, Douglas (Douglas.Swartzendruber@pepperdine.edu)
Date: Thu Oct 18 2001 - 17:40:32 EST

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Thanks, Calman, for the subtley found in your rejoinder; FACS is a brand
name, the CS represents cell sorting which few folks do, and the technology
is flow cytometry.

-----Original Message-----
From: Calman Prussin [mailto:CPRUSSIN@niaid.nih.gov]
Sent: Thursday, October 18, 2001 7:46 AM
To: cyto-inbox
Subject: RE: intracellular IL-5 vs IL-4

Try a Pubmed search using "flow cytometry" rather than FACS, there are many
papers.

We have worked extensively with anti- human IL-5 ICS. See Prussin C. and
D.D. Metcalfe. 1995. Detection of intracytoplasmic cytokine using flow
cytometry and directly conjugated anti-cytokine antibodies.  J Immunol
Methods 188: 117-128. or Elsen L., T. Nutman, D.D. Metcalfe and C. Prussin.
1995. Flow cytometric analysis for cytokine production identifies Th1, Th2,
and Th0 cells within the human CD4+ CD27- lymphocyte subpopulation.  J
Immunol 154:4294-4301.

	I wonder if others have tried this with similar negative results?
Has anyone found higher IL-5 than IL-4 by FACS?

	I would appreciate any comments or references.

	Stan

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