At 12:46 10/10/01 -0400, you wrote: > >Hi All, > >I have a B/D FACScan and two Coulter XLs. I'm looking for the quintessential multi-level >fluorescent bead product to do linearity and sensitivity, compatible with both Coulter >and B/D. > >Thanks in advance > > Hello Deborah, I have used Sphero rainbow calibration particles from Spherotec inc. (cat no. RCP-30-5A) on our ELITEs with success they have 8 fluorescence peaks (of which I can resolve 6 on a good day). I have used them as a one-off to check linearity/sensitivity and have also 'spiked' them into samples as an internal standardisation control -if anyone would like a copy of the excel formula that processes the values they can contact me directly. Best regards, Arnold.
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