Michael -- You might want to use some slides from my lecture 10 on cell cycle at http://stem.utmb.edu/98pth6311 . I hope you find them helpful. - -Jim Leary James F. Leary, Ph.D. Chief, Molecular Cytometry Unit, Div. Infectious Diseases Professor of Internal Medicine, Pathology, Microbiology & Immunology, Biophysics, Human Biological Chemistry & Genetics, and Biomedical Engineering 4.216 Mary Moody Northen Pavilion - Route 0435 University of Texas Medical Branch 301 University Blvd. Galveston, Texas 77555-0435 Tel: 409-747-0547; Fax: 409-747-0550 -----Original Message----- From: Michael Egan [mailto:EganM@war.wyeth.com] Sent: Thursday, October 04, 2001 3:15 PM To: cyto-inbox Subject: Help with presentation Hello fellow flowers: I have been asked to give a 20-30 minute presentation on Flow cytometry to fellow scientist here in our company. The purpose of the presentation is to foster a wider understanding of technology available to research scientists. My target audience will be fellow scientists who are currently not using flow cytometry. The point is to make them more aware of what they could potentially do with flow to further their research. I plan to start out with a basic intro to flow cytometry, the principles and instrumentation. Next I want to give real world examples of the type of analyses one can do using the instrument, such as basic surface staining, intracellular cytokine analysis, and soluble MHC Class I tetramer staining. This is easy for me since this is what I routinely use the instrument for. However, these types of analyses are not the most appropriate for my target audience, because if they needed to do ICC or tetramer staining, they would be in my group and they would already know how to do it! I need to present approaches I'm not currently using such as DNA analysis, cell cycle analysis, sorting, cytokine bead array, etc. If anyone has any Powerpoint slides (or knows of a source of slides) describing the use of flow cytometry to do cell cycle analysis, DNA analysis, cytokine bead arrays, or anything outside of the ordinary surface staining, I would greatly appreciate if you could e-mail me a copy. Any and all help I can get would be greatly appreciated and would help to spare what little is left of my weekend. Thanks again. Michael A. Egan, Ph.D. Senior Research Scientist HIV Vaccine Research and Development Team Wyeth Lederle Vaccines Bldg. 180 Room 258-14 401 North Middletown Road Pearl River, NY 10965 Office: 845-602-3036 Lab: 845-602-4649 Fax: 845-602-4977 eganm@WAR.Wyeth.com
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