Isolation of lymphs from murine liver

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Does anyone have a few helpful techniques for high recovery of lymphs from
mouse liver?

Currently, a perfused liver is mashed through a sieve then treated
enzymatically with collagenase IV and DNAse(both for 15 min.). The
homogenate is then centrifuged slowly to remove the large particulants. A
Percoll gradient is then used to isolate the lymphs. Unfortunately, the
yield and purity are too low for flow cytometry analysis.

Any suggestions?

Regards,

Paul L.Hallberg
KCC CORE Flow Cytometry Facility
Thomas Jefferson University

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