Jim, We study both human into mouse as well as mouse into mouse transplant models and we use the Pharmingen CD 45.1 or CD 45.2 products from them. These are the A20 and 104 antibodies(I forget which is which). These might miss some early erythroid cells as well as all late erythroid cells, but they seem to be on most of the other cells. Mice we have studied are C57BL6, Ly5A, RAGG, Nod SCID, and Nod SCID beta 2 knockout mice as well as a few others. One minor problem in getting the true percent of postive cells is the level of garbage and residual red cells that may sneak into the size gate and thus reduce the percent positive. Anyway-the antibodies we use seem to work fine. Dave Flowers Fred Hutchinson Cancer Research Center "Houston, Jim" wrote: > To All: > > Our lab is using the mouse as a transplant model for human stem cells. We > need to be able to distinguish between Human and Mouse cells after spleen/Bm > have been harvested. The mouse CD45 we use from Pharmingen does not label > all the cells of mouse orign (at least we think not). A fellow researcher > suggested a Sigma Product(Clone M1-9.3HL), but Sigma has discontinued this > reagent. IF you know of a clone that reacts with close to 100% of the > Mouse cells please let me know. > > Jim Houston
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