Hello, I can give you my experience about 2 FACSVantage SE with turbosort option without any judgment on other cytometers from different companies. I practice FACSVantage Turbo since 1996. Regarding cell sorting on immunostaining or GFP labelled cells I could give you reproductible results : On a 30 % starting lymphocyte population double labelled FITC/PE with a speed around 18000 cell/sec we have, after 5 sorting hours, a 70% recovery of a always >99.5% purity population (with a majority >99.8% purity). These sorters are really simple to use. They just need an each week sorting to be really in good conditions. They don't love holidays... And I'm the only operator on the system. Regarding microbiology, we've done good experiments with these apparatus although we had some little problems with noisy scatters properties. Hope it helps you. C Duperray
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