CTLs by flow cytometry

From: Jose Benito (jbenito1@hotmail.com)
Date: Wed Sep 26 2001 - 05:32:12 EST




Dear flowers, I am using flow cytometry to study the level of anti-HIV cytotoxic T cells using an IFN-production assay. Currently I am using two peptides (one from Gag and one from Pol, HLA-A2 restricted) as stimuli, but I am interested in using a large pool of defined epitopes (8 aminoacids in length) in order to be able to study this response in a large population of patients. Does anyone have an idea on how can I get this pool of peptides?
Many thanks
Jose Miguel
Madrid
Spain


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