Cell surface display is a powerful tool for protein analysis. As part of a fusion protein we were able to display WT GFP on the cell surface of Pichia pastoris. Under the fluorescence microscope the fusion is clearly visible as green fluorescence halo around the cells. Attempts to detect and sort the yeast cells on a FACSvantage machine were unsuccessful. We were not able to see any difference between negative and positive control cells, possibly due to auto fluorescence. What kind of settings should be used for the FACS machine and has anybody successfully sorted yeast cells using the WT GFP from Aquorea victoria? Thanks for your contributions, Dominik Stoll, PhD Senior Scientist Twinstrand Therapeutics www.twinstrand.com 8081 Lougheed Mall Burnaby BC, Canada V5A 1W9 tel: (604) 415 7183 fax: (604) 4157185
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