Re: P-glycoprotein

From: Richard Haugland (richard.haugland@probes.com)
Date: Mon Sep 17 2001 - 20:28:33 EST

Next message: Kathy: "preservative"
Previous message: Amy Nield: "Bacterial Counts on Flow"
In reply to: Jones, Patricia: "P-glycoprotein"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Calcein AM is commonly used for this assay by flow cytometry.

http://www.probes.com/media/pis/mp13180.pdf
http://www.probes.com/handbook/figures/1513.html

PGP pumps out unhydrolyzed calcein AM from cells, resulting in decreased
fluorescence of MDR cells relative to non-MDR cells.  The assay uses FL1
in a single-color mode.

Some other AM esters also work:

http://www.probes.com/handbook/sections/1505.html

"Jones, Patricia" wrote:

>
>
> Is anyone aware of a reference lab that tests for P-glycoprotein (the
> MDR gene) by flow cytometry?
>
> Trish Jones
> Flow Cytometry Lab
> UPMC / 763-Scaife Hall
> Pittsburgh, PA 15261

Next message: Kathy: "preservative"
Previous message: Amy Nield: "Bacterial Counts on Flow"
In reply to: Jones, Patricia: "P-glycoprotein"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:31 EST