More about cytoplasmic staining.

From: Finney, Simon (s.finney@ic.ac.uk)
Date: Wed Sep 12 2001 - 04:52:30 EST 

Dear all,

There has been a bit of discussion about intracellular staining recently
with primary conjugated antibodies.

I was trying to prove specificity of my intracellular binding by blocking
with unconjugated antibody first. My conjugated antibody still appears to
bind but I am unclear as to appropriate doses to block with and whether the
conjugated one can swap places with unconjugated one during the second
incubation.

Is this a control too far ?

Isotype controls (admittedly from a different company) show no binding.

Thanks

Dr Simon Finney
Unit of Critical Care
NHLI at Imperial College
London UK

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