Ernst, human ficoll isolated PBMC's also contain monocytes. Assuming you plan not to gate on those - ie. you're only looking at lymphocytes - you can expect ~65 +/-5% CD3+ cells (healthy controls of course). in my experience ficolling tends to reduce number of NK cells fraction but CD3+ cell counts seem unaffected. finally if you are gating on everything - IE. all your PBMC's, you can expect great variation in CD3 %tages. --- Ernst Boehm <eboehm@edv2.boku.ac.at> wrote: > > Dear colleagues, > > I would like to know how many percent of PBMC (isolated from human > blood > via Ficoll gradient centrifugation) are CD3+ ?! ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Get email alerts & NEW webcam video instant messaging with Yahoo! Messenger http://im.yahoo.com
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