chemotaxis

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Dear Dr. Kierulf,

we use a flow cytometric approach
http://www.ma.uni-heidelberg.de/inst/ikc/ikc-fd-hema-immu.html#Chemotaxistes
t, also available by
http://www.orpegen.com/
By using specific chemokines specific cells migrate through the membrane.

Aggregation may be due to the freezing-thawing process (no nitrogen in the
tube?). We use like many others 95% FBS, 5%DMSO and a simple freezing box
from Nalgene.  Thawing (dilute tube content into 50 ml warm medium) and
washing must be quick, dead cells liberate the very sticky DNA.

Regards

Dr. med. C. Thomas Nebe
Facharzt für Laboratoriumsmedizin
Universitaetsklinikum Mannheim
Zentrallabor
Theodor-Kutzer-Ufer 1-3
D-68167 Mannheim
Tel.  +49 621 383-3485
        +49 621 383-2222 (secretary)
FAX  +49 621 383-73 3485 (PC)
        +49 621 383-3819     (paper)
e-mail: thomas.nebe@ikc.ma.uni-heidelberg.de

http://www.ma.uni-heidelberg.de/inst/ikc/  (Internet)
http://pandora/inst/ikc/  (Intranet)

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