Hello all... We have an investigator at NIH who wants to sort the stem cell-containing side population (SP) fraction of Hoechst-labeled bone marrow cells that have been previously labeled with tritiated thymidine, then do scintillation counting on the resulting sorted cell fractions. Previous attempts to use immunodetection of BrdU to detect cycling in these cells did not give desired sensitivity. Obviously, we can't do this sort on our standard Vantage SE - inadequate aerosol containment, a desire not to crud up our instrument with tritium counts, and a lab space that is not certified for radioactivity all prevent this. Since they need a UV excitation source, a "closed" benchtop sorting system such as the FACSCalibur sort module will also not work. Is anyone aware of any laboratory that is set up to sort radioisotope-tagged cells (preferably in the Washington, D.C area, although any lab will do)? Thanks in advance. Bill Telford DTIB-CCR-NCI-NIH
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