Check out: Eur. J. Immunol. 2000. 30: 2612?2619: It seems that in murine dendritic cells, MHCII cross-linking initiates apoptosis of the APC, a phenomenon counteracted by CD40 ligation. Maybe this is what you observe with the anti-HLADQ beads on the B cells? Also (you will probably have done it, but it's worthwhile mentioning) it's important to wash out the azide from the beads. "Acquarola, Nick" wrote: > Does anybody know whether the HLA-DQ molecule has any association with > apoptosis? > We use the Dynal Dynabeads to isolate B cells for microlymphocytoxicity > reactions and have noticed an abnormally higher (dead cell) background. > One thought put to me was that the beads attached to the DQ molecule may > initiate apoptosis. > > Any thoughts? > > Nick Acquarola
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