Sheath fluid for cell sorting Recently I was told that HBSS is preferable to regular PBS based sheath fluids. It is supposed to increase viability in sorted cells. Our problem is that HBSS is buffered with Na-Bicarbonate, it also contains glucose. If we made up a 10-liter batch and let it sit, it would turn alkaline. With the glucose as a nutrient there is also an increased risk of growth. We would like to know what the general opinion is on this. Ole J. Bergh, Supervisor Flow Cytometry Facility University of Pittsburgh Cancer Institute 200 Lothrop Street W 1009 Biomedical Science Tower Pittsburgh, PA 15213 Phone:412 624 0399 Fax: 412 624 9624 <mailto:berghoj@msx.upmc.edu> mailto:berghoj@msx.upmc.edu <http://pci.upmc.edu/internet/flowcyto/flow.htm> http://pci.upmc.edu/internet/flowcyto/flow.htm <http://pci.upmc.edu/internet/flowcyto/flow.htm> -- End --
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