Hey Ken Flowjo does a great job of this, you can generate ratio parameters and plot them as line graphs against time. You can also use Ray Hick's FACS Assistant to generate ratios and line graph data that you can plot in another program like cricket graph or excel, I think you can download it from www.FCSPress.com. I'll brace myself for the barrage of "out of office replies". Is it just me is it very annoying? Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> Ken Field <kfield@mmri.mater.org.au> - 8/21/01 12:57 AM >>> Hi Flowers, I've just started doing some Ca flux experiments using Fluo-3 and Fura Red. Does anyone have any comments and/or suggestions on the software available to calculate the Fluo-3/Fura Red ratio. We're acquiring our data on a BD FACSCalibur thru cellquest. Thanks, Ken Field Mater Medical Research Institute Brisbane, Australia
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