Dear Victor, Negative CD45 is a relatively common finding in childhood ALL. According to the experience of the European BIOMED CA on MRD (see the current issue of Leukemia 2001, 15, 1185-1192) it was found in approx. 15% of cases, which is consistent with previous studies. This is a good feature to follow in MRD studies. Kind regards Anna At 13:10 2001-08-17 -0500, you wrote: >Dear Anna: I would appreciate your help. I've got this morning another of >those very rare cases of pedi ALL with only 17% (dim) positive CD45 (BD Ab). >I would like to know if you could share your views about the above with me >and the FLOW audience at large, for further discussion. We have seen this in >our lab, but rarely. Everytime something unusual comes, I jump and get >nervous. >I know it's not technical error in this case. >Patient is 14 years, diagnosed with L1ALL in Sept'99, 76 % blasts; could'nt >do FLOW, but cytospins showed Tdt+, CD10+, CD19 equivocal. The MPO, double >esterase and Sudan Black were negative. BM chromies showed >47,XY,+X,add(21)(q22). The CFS has shown blasts on/off. He's received >chemotherapy. >Yesterday he returned for chek-up and shows pancytopenia without blasts, but >the BM sohws 54% blasts. We could do BM FLOW that discloses CD45 17% >(only!!), HLADR 91%,CD10 87%, CD19 82% and CD34 77%. The CD5 and Glycophorin >are 5 and 16%. >So, I'm looking for supportive evidence/experience that there are some >CD45-ALLs, and if so, ?how many/how often. >Thank you Anja and to those that may respond. >Victor A. Saldivar-- San Antonio, TX > Anna Porwit Hematopathology Lab. Department of Pathology, Radiumhemmet Karolinska Hospital, 171 76 Stockholm, Sweden Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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