Hello Group, Does anyone have experience using SYTO16 dye from Molecular Probes? I've recently started using it and have found not only radiant green fluorescence, but BRIGHT peaks in FL2, 3 and 4 as well! Not a desirable characteristic when I have a second cell population of interest stained with PE-Cy5. I've tried staining two cell types: a tumor cell line and freshly-isolated leucocytes, with identical results. This phenomenon is present when cells are stained at concentrations from 5uM to 1uM. All signal is lost with concentrations in the 10^-7 order of magnitude. Regards, Tom. ________________________ Thomas P. Ahern University of Vermont/ Vermont Cancer Center E-315 Given Building Burlington, VT 05405 (802) 656-2218 tahern@zoo.uvm.edu
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