Jim, you cannot use PE on a fluorescent scope. I know this is true for confocal, and am pretty sure it is true for any fluorescent microscopy. It bleaches too quickly. Three colors you can use are FITC, Cy3 and Cy5. Pay attention to the filter cubes, because there is spectral overlap between Cy3 and FITC on one end, and Cy5 on the other. Also, you have too have a camera and imaging system to "see" Cy5, as it is barely visible with the naked eye. ron on 8/10/01 12:33 PM, jim phillips at jphillip@med.miami.edu wrote: > > Hello to everyone, > This is a little off the subject of flow... but I have an investigator > that want to do 3 color cell surface fluorescense on a microscope. The > question is .. what three fluorochomes can she use.. The microscope is > just a normal fluorescense scope. I know FITC and PE but is there a > third?? Can you use Cychrome??? She wants to view all three at the > same time.. any help would be appreciated.. Thanks... > Jim Phillips > University of Miami School of Medicine. > > ps.... I just finished my music CD .. entitled ... "222.... 2 old? 2 > fat? 2 ugly?" You can listen to it on mp3.. > > www.mp3.com/jim_phillips > Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.gov
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