Hi Jim, The number of colours you can do on a straight fluorescence microscope is three (with no extra attachments) - UV excitation (e.g. H33342/DAPI/Indo-1; blue/green excitation (e.g. FITC/Oregon Green/Fluo-4); orange fluorescence - TRITC/PI - NOT PE - this normally photobleaches quickly. Other colours can be squeezed out using a CCD camera include Cy5 - which cannot be observed with the naked eye. Hope this is of some help. Gary Warnes ICRF FACS Lab London, UK. jim_phillips wrote:- This is a little off the subject of flow... but I have an investigator that want to do 3 color cell surface fluorescense on a microscope. The question is .. what three fluorochomes can she use.. The microscope is just a normal fluorescense scope. I know FITC and PE but is there a third?? Can you use Cychrome??? She wants to view all three at the same time.. any help would be appreciated.. Thanks... Jim Phillips University of Miami School of Medicine.
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