Flow Cytometry Enthusiasts, My question does not (directly) relate to Flow Cytometry, however I assume many of you out there have home-grown some monoclonal antibodies before. My question specifically deals with the Hollow Fiber Bioreactor technology. We routinely grow large amounts of antibody from suspension hybridomas in a few different systems and have had very good success. We use systems from Cellex Biosciences (now Biovest, Inc.), Fibercell Systems, and Heraeus. Our problem lies in the fact that strictly adherent cell lines (eg Cho, BHK, 293, etc.) do not flourish as well as hybridomas in the Hollow Fiber technology. A few companies have (or will soon) come out with adherent cell line-specific bioreactors, but I still have not found a good one. We want to be able to grow adherent cell lines in the bioreactors without the aggravation of trying to adapt them to suspension status and possibly decrease secretion rates. Has anyone used HF bioreactors to grow adherent cell lines? I would appreciate any information, successful or unsuccessful attempts. Thanks, Ryan Ryan Duggan University of Chicago Immunology Applications Core Facility Flow Cytometry Facility Telephone: (773)702-7293 Email: rcduggan@midway.uchicago.edu
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