Greetings to all on the list Anyone had a go with Pharmingen's DimerX product yet? It's recombinant soluble dimeric mouse class I molecules fused with mouse Ig that can be loaded with peptide to detect antigen-specific T cells ala MHC tetramers. I'd like to hear from anyone who's used it with mouse spleen and lymph node preps to detect antigen-specific CD8 cells. The class I dimers are picked up with an anti-mouse Ig conjugate--looks like it works great with T cell clones but what about when B cells, macs and DCs (lots of surface Ig and Fc receptors) are in the prep? Can all that background binding be successfully blocked with confidence to leave only the peptide/MHC-binding cells? Thanks Matt
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