Good afternoon! I have a question to pose to the general flow community. I have no experience in sorting human cells. However, I now have an investigator who would like to sort a human glioblastoma cell line, a class I biohazard, that has not been screened for hepatitis B or HIV. I've reviewed the common thread for sorting primary human primary cells and found that a number of flow operators did not recommend sorting primary human cells, class I, without some type of aerosol containment. According to the HHS publication, Biosafety in microbiological and biomedical laboratories, class I cell lines pose minimal potential hazard and no special containment equipment is required. So I thought I would just seek the advise from those who sort class I human cell lines. Are there any precautions that I should entertain in protecting myself and other labmates while sorting these cell on my MoFlo which is not specially equipped for biohazardous specimens? Thanks in advance! Julie Oughton Oregon State University
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