Dear Flow-ers,
I recently read the paper "The impact of duration versus extent of
TCR occupancy on T cell activation: a revision of the kinetic proofreading
model" by Rosette et al in Immunity Vol 15, July 2001.
Here they describe intracellular staining for phospho-Jun and phospho
MAPK, after T cell activation.
I would like to reproduce this. Has anyone here experience with doing
this? What are the pitfalls? Also, the autohors describe unconjugated
primary antibodies followed by biton secondary and strep-PE. Anyone know
where I can obtain a FITC-conjugated anti-MAPK that would work for this?
The clones they use are:
anti-phospho-p44/p42 MAPK (E10)
anti-phopspho JNK (G-7)
What other phospho-antibodies work well for this?
Regards,
Dr. Meenakshi Roy
UCLA
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