Wash post Fc block?

From: Thomas Ahern (Thomas.Ahern@uvm.edu)
Date: Thu Jul 12 2001 - 09:26:53 EST

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Hi List,

I'm planning on adding an Fc-block step to my labeling scheme.  I plan to
use heat-inactivated human AB serum at 2% for 15 minutes at room
temperature.  Once this step is complete, should I just pellet the cells
and come on with my mAbs or should I wash the cells a few times first?

Regards,
Tom.

________________________
Thomas P. Ahern
University of Vermont/
Vermont Cancer Center
E-315 Given Building
Burlington, VT 05405
(802) 656-2218
tahern@zoo.uvm.edu

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