Dear Flowers: We have a problem to measure cytokine producing CD4 cells. After stimulation with PHA or PWA with ionomycin, it is hardly to detect CD4 cells. Any one has good resolutions for this problem regarding stimulies and incubation time (we use PWA + Ionomycin for 6 hours and monocin for the last two hours)? Best regards, Yu-Min Huang Division of Neurology Karolinska Institute Huddinge University Hospital 141 86 Stockholm Sweden Tel: +46 8 58582274 Fax: +46 8 58582270
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