Dear List, I recently tried using 5-(and 6-) carboxyfluoresceine diacetate, succinimidyl ester (CFDA/SE) for flow cytometric detection of esterase active bacteria in water samples (ie. as an indication of bacterial viability). Unfortunately, for various reasons, the assay was unsuccessful. I am currently searching for another method for detecting esterase active bacteria. I have read many papers that have successfully used carboxyfluoresceine diacetate (CFDA), although I am a bit reluctant to try this as it is a similar compound to CFDA/SE which I have already had trouble with. I am also thinking of trying ChemChrome B and/or ChemChrome V6, although I have been told by the manufacturer (Chemunex) that they wont sell these reagents unless they are to be used on their own instrumentation. Therefore, I would appreciate hearing from anyone who has successfully used any of the reagents mentioned above. I would also like any comments/suggestions for other compounds that people may have used for the detection of esterase active bacteria. Regards, Daniel Hoefel E-mail: daniel.hoefel@sawater.sa.gov.au ************South Australian Water Corporation disclaimer************ "This e-mail and any attachments to it may be confidential and/or subject to legal professional privilege. If you are not the intended recipient you may not disclose or use the information contained in the message in any way. If received in error please delete all copies and contact the sender by return e-mail. No warranty is made that any attachments are free from viruses. It is the recipient's responsibility to establish its own protection against viruses and other damage."
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