viability of P-19 embryonic carcinoma after sorting

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Did you try using conditioned medium?  Also, try cloning a cell line that
you know grows well.  It's always possible that your cells aren't making it
into the wells for any number of reasons.

David McFarland
GlaxoSmithKline
---------------------- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on
07/09/2001 09:23 ---------------------------


lssharp@wicc.weizmann.ac.il on 01-Jul-2001 09:11



To: cyto-inbox
cc:
Subject:  viability of P-19 embryonic carcinoma after sorting



Hello list members

I wonder if anyone has any ideas on how to make
P-19 embryonic carcinoma cells grow after sorting
into 96 or 24 well plates.
Less than 1% of the sorted cells develop into colonies.

Thanks
Ayala
-------------------------------------------------------------------
Dr. Ayala Sharp
Biological Services,
Weizmann Institute of Science,
Rehovot, ISRAEL.
FAX: 972-8-9344194
Tel: 972-8-9342721 / 972-8-9342235
E-mail: ayala.sharp@weizmann.ac.il

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