In response to the question by Jan Hesketh on cell cycle of yeasts - it is sufficiently different to regular mammalian cells that we had a special unit written on this for Current Protocols in Cytometry by Professor Filipe Sansonetty of the University of Porto, Portugal. the Unit is 11.13 Cell Cycle Analysis of Yeasts and more information can be found at the CPC website http://www.wiley.com/legacy/cp/cpc/ Regards J. Paul Robinson Editor, Current Protocols in Cytometry On 4 Jul 2001, at 14:57, Jan Hesketh wrote: I have had an enquiry from someone in the brewing industry regarding flow cytometry methods for yeasts. They are specifically interested in Cell cycle analysis with PI, Viability with PI/ Fluorescein diacetate, Intracellular pH, Neutral lipids with Nile Red?, Glycogen with acriflavin, Mitochondria with Rhodamine 123, flocculation with ConA-FITC lectin. I have had no experience with any of these areas and was hoping that someone out there may be able to point me in the right direction. Any tips or references would be much appreciated. Thanks, Jan Hesketh Molecular Medicine University of Auckland phone 09 373 7599 ex 4492 fax 09 373 7492 J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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