Re: CD20 negative leukemia

From: Frederic Preffer (preffer@helix.mgh.harvard.edu)
Date: Thu Jun 28 2001 - 21:03:35 EST


John
Tell your pathologists to cheer up and dont waste anymore CD20; sIg- CD19+
tumors with variable to little expression of CD45 is not that unusual.

F Preffer

 At 03:46 PM 6/27/2001 -0500, JOHN MARIO GONZALEZ _ PROFESOR DPT.
MICROBIOLOGIA-. wrote:
>
>Hi colleges:
>
>We have a patient, a woman of 35 years old with 85,500 WBC/ul reported
>as 97% blasts. Flow cytometry showed in SSC Vs CD45 most of the cells
>with low complexity and all of them is an smear in CD45 between
>negative, low and intermediate positive cells never reach the mature
>CD45 high position. These population is rather similar in markers CD19+,
>CD22+, CD20-, CD10-, CD34 -, sIg- and expressed CD5+ intermediate.They
>are 90% of the BM. Patologist were not happy with CD20 and repeatly was
>negative using adequate controls for the anti CD20 mAbs. T and myelod
>markers are negatives.We are wating for the biopsy.
>
>Suggestions ?
>
>JOHN MARIO GONZALEZ M.D., Ph.D. Facultad de Ciencias Pontificia
>Universidad Javeriana Phone 00 571 2850503 Fax   00 571 3208320 Ext 4085
>


Frederic I. Preffer
Department of Pathology
Charlestown Navy Yard- 7140
149 13th Street
Massachusetts General Hospital -East
Charlestown, MA 02129

voice [617] 726-7481
   fax [617] 724-3164



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