Ca2+ in buffers for calcium flux assays

From: Gina_Graziani-Bowering@hc-sc.gc.ca
Date: Tue Jun 26 2001 - 16:09:23 EST

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Dear Flowers,

     I am hoping that someone can clear up some confusion I have regarding the
presence/absence of calcium in buffers used to load cells in a calcium flux
assay. I have one protocol which says to use RPMI  with 0.42 mM Ca2+.  I have
seen other protocols in which chelators are added to the buffers to remove
calcium .  Why the difference?

Thanks,

Gina

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