> Hi: > > I wanted some feedback on the FLOW Jo application. I >currently do all of my analysis with CellQuest. I am wondering if FLOW Jo >may expedite my analysis. However, I do not use the same markers all the >time, and in one experiment I may be running more than one stain with >multiple different antibodies. Basically, very seldom do I run the same set >of markers. How does FLOW Jo work for such conditions?? How about plot >analysis and excel: apparently Excell FLOW Jo puts the data directly into >EXcell?? What has been your experience with FLOW Jo?? Any information would >be useful. > > denise I have been using FlowJo since it was released in 1997. I haven't used CellQuest since I started using FlowJo. I have analysed thousands of samples with a lot of different stain combinations and FlowJo has worked very well for this. In FlowJo you handle multiple stain combinations by grouping all the samples with the same stain and then applying an analysis to all the samples (and you can also make sample-specific adjustments very easily). FlowJo can export statistics for each group and then you simply open them in Excel. This features has saved me a LOT of time. I would highly recommend FlowJo. I could go on about its benefits but you can search the archives for my previous posts on FlowJo or read the review I wrote at <http://www.biocompare.com/prorev.asp?profrevid=49> for more of my opinions. Better still, download the latest version and ask for a free trial serial number. Make sure you do the tutorial and then test it out on your own samples. If you have any specific questions after you have tried it out feel free to ask. Adrian Smith
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